Lip prints, often referred to as cheiloscopic patterns, are a possible tool in forensic identification because they are exclusive to each person and do not alter over time. In order to appraise gender-specific characteristics and determine their relevance for personal identification, this study compares the lip print patterns of males and females. Lip prints were categorized using Tsuchihashi's classification after a sample of 50 people—25 men and 25 women—was gathered using a systematic procedure. A statistical analysis was conducted on the distribution and frequency of various lip print kinds by gender. According to preliminary results, there is a notable difference in the prevalence of specific lip print patterns between males and females, which may imply sexual dimorphism. These outcomes demonstrate the value of lip print analysis as a non-invasive, cost-effective aid in forensic investigations. Further research with larger sample sizes and diverse populations is recommended to strengthen the findings and standardize its application in forensic science. According to the data, males were more likely to exhibit Type III and IV patterns, whereas females were more likely to exhibit Type I and I' patterns. The distribution of Type II was fairly equal for both sexes.